Laccase Activity from Fresh Fruiting Bodies of Ganoderma sp. MK05: Purification and Remazol Brilliant Blue R Decolorization
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چکیده
منابع مشابه
Ligninolytic Enzymes Production and Remazol Brilliant Blue R Decolorization by Tropical Brazilian Basidiomycetes Fungi
Remazol Brilliant Blue R (RBBR) dye was used as substrate to evaluate ligninolytic activity in 125 basidiomycetous fungi isolated from tropical ecosystems. The extracellular RBBR decolorizing activity produced when selected fungi were grown in solid media and in soil contaminated with organochlorines was also evaluated. A total of 106 fungi decolorized the RBBR during the growth in malt extract...
متن کاملPurification and Characterization of Pleurotus florida Laccase (L1) involved in the Remazol Brilliant Blue R (RBBR) Decoloration
Pleurotus florida produces two extracellular laccase (L1 and L2) isoenzymes and the L1 isoenzyme is dominantly involved in the dye decoloration process. L1 isoenzyme was successfully purified to 6.4 fold with a yield of 36% and had a specific activity of 52.6 U mg of protein. The purified laccase was monomeric with an apparent molecular mass of ≈54 kDa. The optimum pH and temperature of the LI ...
متن کاملDecolourisation of Remazol Brilliant Blue R via a novel Bjerkandera sp. strain.
A novel strain of Bjerkandera sp. (B33/3), with particularly high decolourisation activities upon Poly R-478 and Remazol Brilliant Blue R (RBBR) dyes, was isolated. The role of the ligninolytic extracellular enzymes produced by this strain on decolourisation of RBBR was studied in some depth. The basis of decolourisation is an enzyme-mediated process, in which the main enzyme responsible is a r...
متن کاملProduction and Purification of Remazol Brilliant Blue R Decolorizing Peroxidase from the Culture Filtrate of Pleurotus ostreatus.
An extracellular H(inf2)O(inf2)-requiring Remazol brilliant blue R (RBBR) decolorizing enzymatic activity was found in the culture medium of Pleurotus ostreatus. The enzymatic activity was maximally obtained in idiophase, and the optimum C/N ratio was 24. High C/N ratios repressed the enzymatic activity, and addition of veratryl alcohol had no effect on the production of enzyme. The enzyme was ...
متن کاملRemazol Brilliant Blue R Dye Decolourization by Laccase Produced by Pleurotus Sajor-caju via Solid-State Fermentation
The application of synthetic dyes in various industries improved the aesthetic properties of the products, but the discharge of dye-containing effluent into water bodies leads to serious environmental problems. Till date, colour removal from effluent is still a challenge due to the absence of economically attractive treatments. In this study, decolourization of a synthetic dye – Remazol brillia...
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ژورنال
عنوان ژورنال: Journal of Biological Sciences
سال: 2008
ISSN: 1727-3048
DOI: 10.3923/jbs.2009.83.87